Description
Aviva Systems Biology TRH ELISA Kit (Rat) (OKEH03230) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Trh antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Trh and incubated. The Trh found in the sample or standards competes with the biotinylated Trh for limited binding sites on the immobilized anti-Rat Trh antibody. Excess unbound biotinylated Trh and sample or standard Trh is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Trh captured in the well and inversely proportional to the amount of Trh which was contained in the sample or standard